Clinical Laboratory Guide to Services - Minnesota Dept. of Health


The Clinical Guide to Services is a comprehensive reference guide of testing services, shipping information and submission requirements. Efforts have been made to be concise and current with all information, but as testing procedures and regulations change, some information may not be current. Many of these tests are only available with prior approval from MDH. It is impossible to address all situations in this guide, so please contact us with any questions.

Specimen Requirements for Brucella spp.

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Specimen Type
Minimum Volume
Collection Comments
Blood culture Refer to manufacturer’s recommendation Collect appropriate blood volume and number of sets per submitting lab’s protocol. Collect prior to antibiotic use if possible. Multiple specimens increase possibility of obtaining a positive culture.
Blood in sodium polyanethol sulfonate (SPS) 8 ml Blood collected with SPS may be inoculated into blood culture bottles upon receipt at MDH.
Bone Marrow blood culture in bottle fluid 1 ml Collect appropriate bone marrow volume per manufacturer’s recommendation. Some blood culture systems are appropriate for bone marrow.
Bone marrow in sodium SPS 1 ml Bone marrow collected in SPS may be inoculated into culture bottles upon receipt at MDH.
Abscess material


1 ml Collect as needed based on clinical presentation.
Appropriate Postmortem specimen.
Lymph node, liver/spleen biopsy

1-5 g Collect as needed based on clinical presentation.
Appropriate Postmortem specimen.
Synovial fluid, CSF, other body fluids
1 ml Collect as needed based on clinical presentation.
Appropriate Postmortem specimen.
Whole blood 1 ml Collect in EDTA, purple top tube. For molecular testing, must be pre-approved by MDH.
Nasal swab 1 swab For epidemiologic purposes only. Useful only within 24 hours of exposure.
Serum:
Acute and Convalescent
2 ml Acute-phase specimen should be collected ASAP after onset of disease.
Convalescent-phase specimen should be collected >14 days after the acute specimen.
Sent to CDC for testing, must be pre-approved by MDH.

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