Newborn Screening Information for Providers:
Blood Spot Collection
Blood spot collection can be performed by trained personnel such as hospital nursery staff, laboratory staff, or out-of-hospital birth providers.
Timing of blood spot collection is important for accurately interpreting test results. Tests for some of the disorders on the newborn screening panel have different cutoff values based on the infant’s age (in hours) at the time of blood spot collection. As a result, blood spots drawn too early or too late may increase the chance of false positive or false negative results.
Blood spots are best collected between 24 and 48 hours of age. Blood spots collected before 24 hours of age cannot be fully interpreted, which means some test results will be marked “unsatisfactory” on the newborn screening report. An unsatisfactory result will require another blood spot collection. Blood spots collected after 48 hours may not allow enough time for results to be interpreted before serious symptoms occur in newborns affected by certain disorders on the newborn screening panel. However, special circumstances may arise that require blood spot collection at earlier or later times. Please reference the Provider Manual for more information on the timing of bloodspot collection.
The Minnesota Newborn Screening Program uses blood collection standards developed specifically for newborn screening programs by the Clinical and Laboratory Standards Institute.
The primary goal of these standards is to ensure the quality of blood spots collected from newborns. Poor quality specimens place an unnecessary burden on the birth provider, potentially delaying the detection and treatment of an affected infant. If our staff receive a specimen of poor quality, we will request a repeat sample from the birth provider.
Proper specimen collection technique as outlined by the Clinical and Laboratory Standards Institute include the following steps:
- Ensure that the expiration date of the newborn screening card has not passed. Complete the required patient information fields requested on the demographic data portion of the card. Avoid touching the area within the circles on the filter paper section before, during, and after collection of the specimen, since oils and other materials from the hands might affect or contaminate the card or specimen. Do not allow water, feeding formulas, antiseptic solutions, glove powder, hand lotion, or other materials to come into contact with the specimen card before or after use.
- Blood collection from the heel is the standard for newborn screening. The medial and lateral parts of the underfoot are preferred. Blood should never be collected from:
- -the arch of the foot
- -the fingers
- -the earlobes
- -a swollen or previously punctured site
- -IV lines containing other substances (TPN, blood, drugs, etc.)
Neonatal Screening Blood Specimen Collection and Handling Procedure (PDF)
Diagram for proper heel-stick technique
Simple Spot Check (PDF)
Diagram of unsatisfactory specimens
Specimen Collection: Quantity Not Sufficient (PDF)
Newborn Screening Program
601 Robert St. N
St. Paul, MN 55155
For clinics, out-of-hospital birth attendants, and parents who submit specimens infrequently, specimens can be brought to the delivery address above or mailed to the address below. Since high heat and humidity can affect some of the tests, leaving specimens in hot mailboxes or other warm locations should be avoided.
Newborn Screening Program
P.O. Box 64899
St. Paul, MN 55164
Multiple newborn screening specimens may be sent together; however, a shipment should never be delayed or “batched” to wait for additional specimens. Additionally, newborn screening specimens should never be placed in the same container as other specimen types traveling to the Public Health Laboratory.
The following video answers many frequently asked questions about blood spot collection, including the optimal timing of collection, drying of blood spots, and methods of collection. The video was produced in partnership by the Iowa State Hygienic Laboratory and Baby's First Test.
Note: This video is based on Iowa’s newborn screening protocol. Although the video states that a repeat newborn screen should be collected eight weeks after an infant’s last transfusion, we recommend that a repeat specimen be collected 90 days after the last transfusion. Please feel free to contact us with any questions.