Clinical Guide To Services
Infectious Disease Laboratory
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Specimen Requirements for Brucella spp.
The Clinical Guide to Services is a comprehensive reference guide of testing services, shipping information, and submission requirements for Minnesota’s Infectious Disease Laboratory.
Even though there are ongoing efforts to keep information current, some information may not be up to date due to recent changes in testing procedures and/or regulation. Many of these tests are only available with prior approval from the Minnesota Department of Health.
If you cannot find what you are looking for in the guide, please Contact the Infectious Disease Laboratory.
Back to agent: Brucella spp.
|
Specimen Type
|
Minimum Volume
|
Collection Comments
|
| Blood culture | Refer to manufacturer’s recommendation | Collect appropriate blood volume and number of sets per submitting lab’s protocol. Collect prior to antibiotic use if possible. Multiple specimens increase possibility of obtaining a positive culture. |
| Blood in sodium polyanethol sulfonate (SPS) | 8 ml | Blood collected with SPS may be inoculated into blood culture bottles upon receipt at MDH. |
| Bone Marrow blood culture in bottle fluid | 1 ml | Collect appropriate bone marrow volume per manufacturer’s recommendation. Some blood culture systems are appropriate for bone marrow. |
| Bone marrow in sodium SPS | 1 ml | Bone marrow collected in SPS may be inoculated into culture bottles upon receipt at MDH. |
| Abscess material |
1 ml | Collect as needed based on clinical presentation. Appropriate Postmortem specimen. |
| Lymph node, liver/spleen biopsy |
1-5 g | Collect as needed based on clinical presentation. Appropriate Postmortem specimen. |
| Synovial fluid, CSF, other body fluids | 1 ml | Collect as needed based on clinical presentation. Appropriate Postmortem specimen. |
| Whole blood | 1 ml | Collect in EDTA, purple top tube. For molecular testing, must be pre-approved by MDH. |
| Nasal swab | 1 swab | For epidemiologic purposes only. Useful only within 24 hours of exposure. |
| Serum: Acute and Convalescent |
2 ml | Acute-phase specimen should be collected ASAP after onset of disease. Convalescent-phase specimen should be collected >14 days after the acute specimen. Sent to CDC for testing, must be pre-approved by MDH. |
Back to List of Tests