Clinical Guide To Services
Infectious Disease Laboratory
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Last Updated: 03/09/2023
Specimen Requirements for Haemophilus ducreyi
The Clinical Guide to Services is a comprehensive reference guide of testing services, shipping information, and submission requirements for Minnesota’s Infectious Disease Laboratory.
Even though there are ongoing efforts to keep information current, some information may not be up to date due to recent changes in testing procedures and/or regulation. Many of these tests are only available with prior approval from the Minnesota Department of Health.
If you cannot find what you are looking for in the guide, please Contact the Infectious Disease Laboratory.
Back to agent: Haemophilus ducreyi
|Ulcer Swab||Transport tube containing either 1 ml of 2PS or M4/M4RT, PBS, saline, 10 mM Tris-HCl (pH 8.0) or Tris buffer containing 0.1M EDTA and 0.5% SDS, mixed well with the transport medium for 5-10 seconds.
Commercial viral transport medium (reduce the volume to 1 ml), AssayAssure Universal Microbiology Collection kit (Thermo Fisher Scientific) or STD swab specimen collection and transport kit (Roche) can also be used if available.
Store specimens at -20°C or colder prior to shipping . Recommend dry ice be used and ship early in the week (do not ship on Friday), overnight express with cold pack is not preferred.
- Sterile dacron-tipped swab is used by rolling firmly across the base of the ulcer.
- Cleaning before sampling is usually not necessary, unless the ulcers were covered with bloody transudates/pus resulted from secondary infections; if so the surface materials can be gently removed with a wet swab.
- If multiple ulcers exist different swabs should be used to avoid cross contamination from ulcer to ulcer.
- If only a single specimen will be collected select the largest ulcer with exudates.
- Express swab against the side of tube and discard.
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